Role of the T5 gene D15 nuclease in the generation of nicked bacteriophage T5 DNA
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Role of the T5 gene D15 nuclease in the generation of nicked bacteriophage T5 DNA.
The processing of newly replicated concatameric T5 DNA into both single stranded DNA changed of unit length and single-stranded fragments of sizes comparable to those found in mature T5 virion DNA occurs in the absence of late T5 protein synthesis. The formation of unit-length, single-stranded DNA chains does not require the early T5 gene D15 nuclease: however, the subsequent formation of the s...
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15 صفحه اولPolarized injection of the bacteriophage T5 chromosome.
Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first.
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The gene D5 product (gpD5) of bacteriophage T5 is a DNA-binding protein that binds preferentially to double-stranded DNA and is essential for T5 DNA replication, yet it inhibits DNA synthesis in vitro. Mechanisms of inhibition were studied by using nicked DNA and primed single-stranded DNA as a primer-template. Inhibition of T5 DNA polymerase activity by gpD5 occurred when double-stranded regio...
متن کاملThe highly processive DNA polymerase of bacteriophage T5. Role of the unique N and C termini.
The DNA polymerase encoded by bacteriophage T5 has been reported previously to be processive and to catalyze extensive strand displacement synthesis. The enzyme, purified from phage-infected cells, did not require accessory proteins for these activities. Although T5 DNA polymerase shares extensive sequence homology with Escherichia coli DNA polymerase I and T7 DNA polymerase, it contains unique...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1977
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.24.1.177-193.1977